Thin-Layer Chromatography (TLC)

• Applications:

  • Quick identification of active components.

  • Screening of plant extracts for bioactive compounds.

High-Performance Liquid Chromatography (HPLC)

• Applications:

  • Quantitative and qualitative analysis of active compounds.

  • Purity testing and compound isolation.

  • Detection of flavonoids, phenolic acids, and alkaloids.

Gas Chromatography-Mass Spectrometry (GC-MS)

• Applications:

  1. Metabolite profiling of plant extracts.

  2. Identification of volatile compounds.

  3. Analysis of essential oils, fatty acids, and more.

NMR Sample Analysis Charges

 Basic 1H-NMR Analysis

13C-NMR Analysis

• Applications: Detailed carbon skeleton determination, compound characterization.

Advanced 2D-NMR Techniques

• Applications: NOESY, COSY, HSQC, HMBC for complex structural elucidation.

Total Protein Content

• Method: Bradford Assay, Lowry Method, or Kjeldahl Analysis (as per requirement).

• Applications: Nutritional analysis, protein quantification in plant or food samples.

Total Fat/Oil Content

• Method: Soxhlet Extraction or Gravimetric Analysis.

• Applications: Determination of lipid content in seeds, food, or plant materials.

Total Phenolic Content (TPC)

• Method: Folin-Ciocalteu Reagent Method.

• Applications: Assessment of antioxidant potential in plant extracts or food products.

Total Flavonoid Content (TFC)

• Method: Aluminum Chloride Colorimetric Assay.

• Applications: Determination of flavonoids as indicators of bioactivity in plant-based samples.

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