Modules-1 

Extractions of Metabolites 

• Duration: 2 Weeks

• Fee: ₹2,000

• What is the Extraction 

• Different types of Extraction methods

• What are the metabolites and types of metabolites

• Extractions of Primary and  Secondary Metabolites  

• Isolation and Identification of different types of compounds

Modules-2 

Thin Layer Chromatography (TLC)

• Duration: 1 Weeks

• Fee: ₹1,000

• What is TLC

• How to work with TLC

• Data generation through TLC

Modules-3

High- Performance Liquid Chromatography

• Duration: 4 Weeks

• Fee: ₹3,000

• What is HPLC

• Parts of HPLC 

• What is Mobile and stationary phases in HPLC

• Types of Mobile and stationary phases

• Work and types of detectors 

• Types and work of Pumps in HPLC

• Role of HPLC in Metabolic profiling

Modules-4

Gas Chromatography-Mass Spectroscopy

• Duration: 4 Weeks

• Fee: ₹4,000

• What is GC MS

• Why do we use GC-MS

• Component of GC-MS

• How to work  GC-MS

• Sample Preparation for GC-MS

• How to get the GC-MS spectra

• Interpretation of GC-MS spectra

Modules-5

Specialized Training in Nuclear Magnetic Resonance Spectroscopy

• Duration: 5 Weeks

• Fee: ₹5,000

• Topics Covered:

• What is NMR (Fundamentals of NMR)

• Component of NMR

• How to work  NMR and different types of NMR experiments

• 1D NMR  a. Chemical Shift b. Coupling Constant c. Relaxation Phenomena d. Applications 

•  2D NMR a. Principle b. Different methods (COSY,NOESY,TOCSY,HMQC,HMBC,HSQC,etc) c.

• Different types of Multinuclear NMR C-13, N-15, F-19, Si-28, P-31

• Sample Preparation for NMR.

• How to get the spectra? d. When to use these methods? 

• Data interpretation.

•  Solid State NMR 

• Metabolite profiling applications

• Data analysis using advanced software

Biochemical analysis 

• Duration: 5 Weeks

• Fee: ₹3,000

• Total Protein content

• Total fat, oil content

• Total sugar content

• Total phenolic content

•  Total flavonoid content.

• Enzyme assays such as Superoxide Dismutase,Peroxidase, Glutamase reductage , Ascorbate peroxidase, Lipid Peroxidase, and Catalase.

Biological activity (In vitro) 

• Duration: 5 Weeks

• Fee: ₹3,000

• Antioxidant activity by different methods such as DPPH, FRAP, and ABTS

• AntiInflamatory activity

• Antimicrobial activity

• Antidiabetic activity

Hands-on training

• Each student prepares own training report

• Full guidance

• Provided laptop for report writing